It is not uncommon to see some photographers use 40-100 stacked images in macro and photomicrography. The subject depth and magnification determines how many images you need in a stack to get a sharp picture with enhanced depth of field. The depth of field can be as small as 1-2 microns at magnifications of 400x and higher with a microscope. In macrophotography the depth of field can be less than a millimetre and in microscopy the depth of field is measured in microns (thousands of a millimetre). In macro and photomicrography the closer one is to the subject or the higher the magnification the shallower the depth of field. Stacking can be used in landscape photography by combining a photograph focused on the foreground and one focused on the background to create an incredible depth of field that exceeds what could be done using a very high F-stop (F16-F64), using a tilt-shift lens, or even a large format camera. Stacking is done in software using the digital images. Comparing Photoshop, Helicon Focus and ZereneĪbove is a Focus stack of a diatom where I combined 10 images in Photoshop 400XĪ focus stack is a photograph that is made up of a combination of two or more images that were focused at different points and then after combining the stacks the final image has a much greater depth of field.
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